vivit peptide VIVIT selectively and potently inhibits calcineurin/NFAT interaction - waglerin-1-peptide-complex VIVIT selectively and potently inhibits calcineurin/NFAT interaction VIVIT Peptide: A Selective Inhibitor Targeting the Calcineurin-NFAT Pathway

lemon-bottle-skin VIVIT peptide is a highly specific and potent inhibitor that plays a crucial role in research and potential therapeutic applications by targeting the calcineurin-NFAT signaling pathway. This peptide acts by selectively interrupting the interaction between calcineurin and Nuclear Factor of Activated T cells (NFAT), a critical mechanism involved in various cellular processes including immune responses, cell growth, and cardiovascular remodeling. Understanding the molecular intricacies and applications of VIVIT peptide is essential for researchers exploring immune modulation and related disease mechanisms.

The efficacy of VIVIT peptide lies in its ability to bind to calcineurin with high affinity, thereby preventing the dephosphorylation and subsequent activation of NFATNFAT Inhibitor. This targeted approach distinguishes it from other immunosuppressive agents that may have broader effects. Unlike broad-spectrum calcineurin inhibitors such as FK506 or Cyclosporin A (CsA), VIVIT peptide primarily inhibits the calcineurin-NFAT interaction without significantly compromising the phosphatase activity of calcineurin itself or affecting non-NFAT signaling pathways. This specificity is a key advantage, potentially leading to a more favorable therapeutic profile with fewer off-target side effects.

Mechanisms and Applications of VIVIT Peptide

Research has demonstrated various applications and mechanisms of VIVIT peptide. For instance, the dNP2-VIVIT Ameliorates Experimental Autoimmune Encephalomyelitis in studies, highlighting its potential in treating autoimmune diseases. The synthetic peptide dNP2-VIVIT has been investigated for its inhibitory effects on NFAT function in the context of these conditionsDescription.Inhibitor of calcineurin-mediated NFAT activation; Alternative Names. VIVIT peptide.. Furthermore, polypeptide 11R-VIVIT has shown promise in stimulating osteoblastogenesis through inhibition of the calcineurin/NFAT signaling pathway. The 11R-VIVIT peptide is a well-studied variant, known for its cell-permeable nature and its specific targeting of the calcineurin-NFAT interaction.作者：H Noguchi·2017·被引用次数：22—This peptide (11R-VIVIT)selectively interferes with calcineurin-NFAT interactionwithout affecting the activity of calcineurin phosphatase and provides ...

Another important application involves its use in cellular research for identifying NFAT target genes. The VIVIT peptide constitutes a highly selective inhibitor of NFAT, allowing researchers to directly probe the downstream effects of NFAT activation in various cell types. The development of cell permeable NFAT inhibitory peptide Sim-2-VIVIT and forms like 11R-VIVIT has facilitated *in vitro* studies by enabling easier cellular uptake. Studies have also shown that VIVIT inhibited the production of NO and the secretion of IL-12 p70, IL-23, and TNF, suggesting a broad role for NFAT in inflammatory gene expression and indicating VIVIT's potential in managing inflammatory processes.

VIVIT Peptide in Cardiovascular and Other Research

The therapeutic potential of VIVIT, a selective peptide inhibitor, has been explored in cardiovascular disorders. Research suggests that VIVIT selectively and potently inhibits calcineurin/NFAT interaction, which is implicated in conditions like cardiac hypertrophy.作者：H Yu·2006·被引用次数：58—Conclusions—VIVITappears to be a specific and potent inhibitor of NFAT activation and thus of NFAT-mediated proliferation and inflammation. Unlike FK506 or CsA ... Scientists have also investigated the delivery of the VIVIT Peptide to Human Glioma Cells to Interfere with Calcineurin-NFAT Signaling, demonstrating its potential utility beyond immunological applications. In these studies, VIVIT was expressed as a fusion protein to facilitate its delivery and study its intracellular effects11R-VIVIT is a cell-permeable nuclear factor of activated T cells (NFAT) inhibitor. 11R-VIVIT can be used for the research of podocyte and diabetic ....

The optimized core of VIVIT peptide, derived from the conserved "PxIxIT" sequence found in the NFAT family, effectively blocks the interaction between calcineurin and NFATThe cell‐permeable peptide11R‐VIVITbinds to CaN and specifically blocks its ability to activate NFAT, interrupting NFAT activation and translocation. The lack .... This mechanism is crucial for understanding how NFAT regulates effector functions, such as those seen in CD4+ T helper cells. Moreover, Expression of peptide VIVIT in mammalian cells effectively blocks the CN–NFAT interaction and its downstream signaling, reinforcing its role as a specific modulator of this pathway.Specific NFAT Inhibitor (VIVIT-16) The NFAT Inhibitor-1 (VIVIT peptide) is often cited as a superior agent for inhibiting calcineurin-mediated NFAT activation.

For researchers seeking to use this potent inhibitor, several forms are available. The NFAT Inhibitor (VIVIT peptide), often identified by its CAS Number 249537-73-3, is a commercially available reagent with high purity, typically ≥95% (HPLC). The 11R-VIVIT peptide is another frequently utilized variant, known for its cell-permeable properties which enhance its utility in experimental settings. Different product codes and synonyms exist, such as "NFAT regulatory domain" as an alternative name, underscoring the broad research interest in this molecule.The11R-VIVIT peptideis a cell-permeable inhibitor of the nuclear factor of activated T-cells (NFAT), specifically targeting the calcineurin-NFAT interaction. The NFAT Inhibitor is described as a cell-permeable peptide that inhibits calcineurin-mediated NFAT activation, and its molecular weight is approximately 1683How does NFAT3 regulate the occurrence of cardiac hypertrophy?.94 Da. The chemical formula for one form is C75H118N20O22S.Inhibiting the Calcineurin-NFAT (Nuclear Factor of Activated T Cells ...

In summary, the VIVIT peptide is a significant research tool and potential therapeutic lead compound作者：JM Choi·2012·被引用次数：31—A cell permeable form of theNFAT inhibitory peptide VIVIT(Sim-2-VIVIT) was synthesized and showed inhibitory effects on human CD4 or CD8 T-cell activation.. Its precise mechanism of action, selectively interfering with calcineurin-NFAT interaction without broad calcineurin inhibition, offers a unique advantage in studying and modulating pathways involved in immunity, inflammation, cardiovascular disease, and other cellular processes. Continued research into modified versions like 11R-VIVIT and its delivery methods, such as when VIVIT was expressed as a fusion protein, will further illuminate its therapeutic and scientific potential.